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hiscript reverse transcriptase kit  (Vazyme Biotech Co)


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    Structured Review

    Vazyme Biotech Co hiscript reverse transcriptase kit
    Hiscript Reverse Transcriptase Kit, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 99/100, based on 5438 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cdna+reverse+transcriptase+kit/HiScript+III+1st+Strand+cDNA+Synthesis+Kit+%2BgDNA+wiper/pm42816486-356-13-17
    Average 99 stars, based on 5438 article reviews
    hiscript reverse transcriptase kit - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Isolation:

    Article Title: Notoginsenoside R1 ameliorates the inflammation induced by amyloid‑β by suppressing SphK1‑mediated NF‑κB activation in PC12 cells
    Article Snippet: .. Total RNA from PC12 cells was isolated using Biozol reagent (Biomiga), and cDNA was synthesized using a cDNA Reverse Transcriptase Kit (Vazyme Biotech Co., Ltd.) according to the manufacturer's instructions. .. The temperature protocol for reverse transcription was as follows: 37°C for 15 min and 98°C for 5 min. qPCR was performed using SYBR Green Real-time PCR Master Mix (Vazyme Biotech Co., Ltd.) on an Eppendorf Master Cycler ep RealPlex4 (Eppendorf SE) as previously described ( ).

    Article Title: Inhibition of neuronal nitric oxide synthase protects against hippocampal neuronal injuries by increasing neuropeptide Y expression in temporal lobe epilepsy mice.
    Article Snippet: Neuronal nitric oxide synthase (nNOS) plays a pivotal role in the pathological process of neuronal injury in the development of epilepsy.. Our previous study has demonstrated that nitric oxide (NO) derived from nNOS in the epileptic brain is neurotoxic due to its reaction with the superoxide radical with the formation of peroxynitrite.. Neuropeptide Y (NPY) is widely expressed in the mammalian brain, which has been implicated in energy homeostasis and neuroprotection.

    Article Title: ADAM10 suppresses demyelination and reduces seizure susceptibility in cuprizone-induced demyelination model.
    Article Snippet: The metalloproteinase ADAM10 is the most important amyloid precursor protein (APP) α-secretase, preventing the deposit of neurotoxic amyloid β (Aβ) peptide and generating a soluble APP fragment (sAPPα) with neurotrophic functions.. Recent studies have suggested that ADAM10 also play a role in the pathogenesis of inflammatory CNS diseases, such as multiple sclerosis (MS).. Demyelination is the hallmarks of MS but the mechanisms involved remain unclear.

    Article Title: Valproic acid suppresses cuprizone-induced hippocampal demyelination and anxiety-like behavior by promoting cholesterol biosynthesis.
    Article Snippet: The quantitative analyses of the immunostaining were performed using the ImageJ software (NIH, Bethesda, MD, USA) as described in our previous study (Zhu et al., 2017; Zhu et al., 2015). .. Total RNA was isolated with TRIzol reagent (Vazyme Biotech, Nanjing, China) according to the manufacturer's instructions. cDNA was synthesized using cDNA Reverse Transcriptase Kit (Vazyme Biotech, Nanjing, China). ..

    Synthesized:

    Article Title: Notoginsenoside R1 ameliorates the inflammation induced by amyloid‑β by suppressing SphK1‑mediated NF‑κB activation in PC12 cells
    Article Snippet: .. Total RNA from PC12 cells was isolated using Biozol reagent (Biomiga), and cDNA was synthesized using a cDNA Reverse Transcriptase Kit (Vazyme Biotech Co., Ltd.) according to the manufacturer's instructions. .. The temperature protocol for reverse transcription was as follows: 37°C for 15 min and 98°C for 5 min. qPCR was performed using SYBR Green Real-time PCR Master Mix (Vazyme Biotech Co., Ltd.) on an Eppendorf Master Cycler ep RealPlex4 (Eppendorf SE) as previously described ( ).

    Article Title: Inhibition of neuronal nitric oxide synthase protects against hippocampal neuronal injuries by increasing neuropeptide Y expression in temporal lobe epilepsy mice.
    Article Snippet: Neuronal nitric oxide synthase (nNOS) plays a pivotal role in the pathological process of neuronal injury in the development of epilepsy.. Our previous study has demonstrated that nitric oxide (NO) derived from nNOS in the epileptic brain is neurotoxic due to its reaction with the superoxide radical with the formation of peroxynitrite.. Neuropeptide Y (NPY) is widely expressed in the mammalian brain, which has been implicated in energy homeostasis and neuroprotection.

    Article Title: ADAM10 suppresses demyelination and reduces seizure susceptibility in cuprizone-induced demyelination model.
    Article Snippet: The metalloproteinase ADAM10 is the most important amyloid precursor protein (APP) α-secretase, preventing the deposit of neurotoxic amyloid β (Aβ) peptide and generating a soluble APP fragment (sAPPα) with neurotrophic functions.. Recent studies have suggested that ADAM10 also play a role in the pathogenesis of inflammatory CNS diseases, such as multiple sclerosis (MS).. Demyelination is the hallmarks of MS but the mechanisms involved remain unclear.

    Article Title: Valproic acid suppresses cuprizone-induced hippocampal demyelination and anxiety-like behavior by promoting cholesterol biosynthesis.
    Article Snippet: The quantitative analyses of the immunostaining were performed using the ImageJ software (NIH, Bethesda, MD, USA) as described in our previous study (Zhu et al., 2017; Zhu et al., 2015). .. Total RNA was isolated with TRIzol reagent (Vazyme Biotech, Nanjing, China) according to the manufacturer's instructions. cDNA was synthesized using cDNA Reverse Transcriptase Kit (Vazyme Biotech, Nanjing, China). ..

    Reverse Transcription:

    Article Title: Notoginsenoside R1 ameliorates the inflammation induced by amyloid‑β by suppressing SphK1‑mediated NF‑κB activation in PC12 cells
    Article Snippet: .. Total RNA from PC12 cells was isolated using Biozol reagent (Biomiga), and cDNA was synthesized using a cDNA Reverse Transcriptase Kit (Vazyme Biotech Co., Ltd.) according to the manufacturer's instructions. .. The temperature protocol for reverse transcription was as follows: 37°C for 15 min and 98°C for 5 min. qPCR was performed using SYBR Green Real-time PCR Master Mix (Vazyme Biotech Co., Ltd.) on an Eppendorf Master Cycler ep RealPlex4 (Eppendorf SE) as previously described ( ).

    Article Title: Inhibition of neuronal nitric oxide synthase protects against hippocampal neuronal injuries by increasing neuropeptide Y expression in temporal lobe epilepsy mice.
    Article Snippet: Neuronal nitric oxide synthase (nNOS) plays a pivotal role in the pathological process of neuronal injury in the development of epilepsy.. Our previous study has demonstrated that nitric oxide (NO) derived from nNOS in the epileptic brain is neurotoxic due to its reaction with the superoxide radical with the formation of peroxynitrite.. Neuropeptide Y (NPY) is widely expressed in the mammalian brain, which has been implicated in energy homeostasis and neuroprotection.

    Article Title: ADAM10 suppresses demyelination and reduces seizure susceptibility in cuprizone-induced demyelination model.
    Article Snippet: The metalloproteinase ADAM10 is the most important amyloid precursor protein (APP) α-secretase, preventing the deposit of neurotoxic amyloid β (Aβ) peptide and generating a soluble APP fragment (sAPPα) with neurotrophic functions.. Recent studies have suggested that ADAM10 also play a role in the pathogenesis of inflammatory CNS diseases, such as multiple sclerosis (MS).. Demyelination is the hallmarks of MS but the mechanisms involved remain unclear.

    Article Title: Microglia sense and suppress epileptic neuronal hyperexcitability.
    Article Snippet: .. Total RNA was extracted from cultured cells using Trizol reagent (Vazyme, Nanjing, China) and cDNA was generated using the cDNA Reverse Transcriptase Kit (Vazyme). ..

    Article Title: Valproic acid suppresses cuprizone-induced hippocampal demyelination and anxiety-like behavior by promoting cholesterol biosynthesis.
    Article Snippet: The quantitative analyses of the immunostaining were performed using the ImageJ software (NIH, Bethesda, MD, USA) as described in our previous study (Zhu et al., 2017; Zhu et al., 2015). .. Total RNA was isolated with TRIzol reagent (Vazyme Biotech, Nanjing, China) according to the manufacturer's instructions. cDNA was synthesized using cDNA Reverse Transcriptase Kit (Vazyme Biotech, Nanjing, China). ..

    Cell Culture:

    Article Title: Microglia sense and suppress epileptic neuronal hyperexcitability.
    Article Snippet: .. Total RNA was extracted from cultured cells using Trizol reagent (Vazyme, Nanjing, China) and cDNA was generated using the cDNA Reverse Transcriptase Kit (Vazyme). ..

    Generated:

    Article Title: Microglia sense and suppress epileptic neuronal hyperexcitability.
    Article Snippet: .. Total RNA was extracted from cultured cells using Trizol reagent (Vazyme, Nanjing, China) and cDNA was generated using the cDNA Reverse Transcriptase Kit (Vazyme). ..



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